Amid Biosciences offers high-purity BirA Biotin Ligase enzyme, recombinantly expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. This innovative dual-tag architecture allows for effortless, flexible removal of the BirA enzyme following your biotinylation reaction using either Ni-NTA or glutathione agarose resin columns, ensuring a pristine end product.

Our BirA biotin ligase is optimized for highly precise, site-specific protein biotinylation. It is a critical tool for researchers utilizing proximity labeling, AviTag™ sequence targeting, and advanced molecular assay developments that require highly selective covalent labeling of target proteins.

Key Features & Specifications

• Expression Host: Escherichia coli (E. coli)
• Expressed Region: Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)
• Dual-Tag System: N-terminal 6XHis and GST tags (~64 kDa total molecular weight) for streamlined downstream enzyme depletion.
• High Activity: >3,000 Units/µg.
• Activity Definition: 1 Unit is defined as the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in optimal reaction buffer conditions.
• Formulation & Buffer: Supplied in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol.

Applications

Validated for high-performance downstream research applications across in vitro and in vivo platforms, including:

• Site-Specific Biotinylation of AviTag™ or Biotin Acceptor Peptide (BAP) tagged proteins.
• Proximity Labeling and interactome mapping studies.
• Preparation of biotinylated targets for Streptavidin-based pull-downs, ELISAs, and surface plasmon resonance (SPR).

Storage & Handling Guidelines

• Storage Conditions: Recommended storage at -20°C for extended stability.
• Shipping Notice: Product requires shipping on dry ice. Please contact [email protected] for shipping estimates.

Catalog # BBL-G-301 (0.1 mg).
This product is for laboratory research use only (RUO).