RhoA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91169-50, BS91169-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61586(Human) Q9QUI0(Mouse) P61589(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
FC:1:50-1:100

Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: RhoA polyclonal antibody detects endogenous levels of RhoA protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RhoA on rat brain lysates using anti-RhoA antibody at 1/1,000 dilution., ICC staining RhoA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: h12, RHOA, Aplysia ras related homolog 12, ARH12, ARHA, H 12, H12, Oncogene RHO H12, Ras homolog family member A, Ras homolog gene family member A, Rho A, Rho cDNA clone 12, RHO H12, RHO12, RHOA, RHOA, RHOH12, Small GTP binding protein Rho A, Transforming protein Rho A, Transforming protein RhoA,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified