HSP72 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66834-50, MB66834-100

Swiss-Prot: P08107

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of HSP72 protein.

Extra Notes: Western blot analysis of HSP72 expression in HL60 (A) whole cell lysates., Immunofluorescent analysis of HSP72 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Bioworld Molecular Weight: ~ 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: HSPA1; HSX70; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP72. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified