SHH Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80230-50, BS80230-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q62226

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other twofamily members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signalingmolecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles inregulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult,including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shhis synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. Themature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment isthe core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat andgerbil Shh-N, respectively.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 45KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SHH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Alternative Name: 9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M100081

Immunogen: Recombinant fusion protein of mouse SHH(NP_033196.1).

Conjugate: Unconjugated

Modification: Unmodified