Pan Phospho-Tyrosine Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79455-50, BS79455-100

Host: Rabbit

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers. pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Extra Notes: Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb at 1:1000 dilution.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s., Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight., Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

Bioworld Molecular Weight: 20-70KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S &T.

Conjugate: Unconjugated

Modification: Phosphorylated