Shc (phospho-Ser239/240) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES13126-50, ES13126-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011],

Alternate Name: SHC-transforming protein 1 (SHC-transforming protein 3) (SHC-transforming protein A) (Src homology 2 domain-containing-transforming protein C1) (SH2 domain protein C1)

Source: Rabbit

Applications: WB; ELISA; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized phosho peptide around human Shc (Ser239 and 240)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 66 (p66 isoform), 52 (p52 isoform), 46 (p46 isoform)kD

Human Gene ID: 6464

Human SWISS Prot NO: P29353

Subcellular Location: Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; [Isoform p66Shc]: Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.

Research Use Only