SET Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91227-50, BS91227-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01105(Human) Q9EQU5(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC, IP, FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:10-1:50
FC:1:50-1:100

Background: Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SET polyclonal antibody detects endogenous levels of SET protein.

Bioworld Molecular Weight: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SET on K562 cell lysates using anti-SET at 1/500 dilution., ICC staining SET in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: IGAAD, I-2PP2A, TAF-I, SET, 2PP2A, HLA DR associated protein II, HLA-DR-associated protein II, I 2PP2A, I-2PP2A, I2PP2A, IGAAD, Inhibitor of granzyme A activated DNase, Inhibitor of granzyme A-activated DNase, Inhibitor of GzmA-activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPII, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, Protein SET, Set, SET nuclear oncogene, SET translocation, SET translocation (myeloid leukemia-associated), SET, TAF I, TAF IBETA, TAF-I, TAFI, Template activating factor I, Template-activating factor I, Template-Activating Factor-I, chromatin remodelling factor,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified