SUMO4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91302-50, BS91302-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6EEV6(Human) P61957(Mouse) P61959(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, , FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
ICC:1:100-1:500

Background: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2, SUMO-3 and SUMO-4, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Ubiquitin and SUMO proteins utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2, SUMO-3 and SUMO-4 proteins localize to the nucleus. In contrast to the other SUMO proteins, SUMO-4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90, which may impair processing to mature form and conjugation to substrates. It is suggested that defects in the gene that encodes for the SUMO-4 protein may be involved in the pathogenesis of type I diabetes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SUMO4 polyclonal antibody detects endogenous levels of SUMO4 protein.

Bioworld Molecular Weight: 11 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution. Positive control:
Lane 1: 293T
Lane 2: Jurkat, ICC staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SUMO-4, SUMO4, dJ281H8.4, IDDM5, Small ubiquitin like modifier 4 protein, Small ubiquitin-like protein 4, Small ubiquitin-related modifier 4, SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3H4, SUMO 4, SUMO-4, SUMO4, SUMO4,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified