NeuN Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67571-50, BS67571-100

Swiss-Prot: A6NFN3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of NeuN protein.

Extra Notes: Western blot analysis of NeuN expression in U2OS (A), mouse testis (B), rat testis (C), rat muscle (D) whole cell lysates.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NeuN. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified