Bax Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10330-50, MB10330-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q07812

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 21 kDa; Observed MW: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bax in C6, 3T3, Hela lysates using Bax antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Bax antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4

Immunogen: A synthetic peptide of human Bax

Conjugate: Unconjugated

Modification: Unmodified