HCoV-OC43 Spike S2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78085-50, BS78085-100

Swiss-Prot: P36334

Host: Rabbit

Reactivity: HCoV-OC43

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Human coronavirus Spike Protein , using HCoV-OC43 Spike S2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Bioworld Molecular Weight: 75KDa/145KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: A synthetic peptide of coronavirus Spike S2(YP_009555241.1).

Conjugate: Unconjugated

Modification: Unmodified