Human KLK10 (Kallikrein 10) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1110-48, ELK1110-96, ELK1110-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

Assay Type: Competitive Inhibition

Sensitivity: 48.8 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Tumor immunity; Reproductive science;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O43240

Research Use Only