Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail

Sizes: 20 Tests, 100 Tests, 100 Tests×2

Catalogue Numbers: E-AB-FC0010-20, E-AB-FC0010-100, E-AB-FC0010-100×2

Citations, Manuals and MSDS Available upon request.

Abbreviation:

Target Synonym:

Conjugation: FITC, PE, PE/Cyanine7, PerCP

Human

Application: FCM

Usage: For whole blood samples, add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
For other samples, 1×10^6 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.

Isotype:

Clone No.: OKT-8, RPA-T4, OKT-3, HI30

Background: This product is a FCM antibody cocktail made up of FITC Anti-Human CD8a Antibody [Clone: OKT-8] (Mouse IgG2a, κ), PE Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ), PE/Cyanine7 Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ) and PerCP Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).;
CD8 is mainly expressed on cytotoxic T cells and also some subpopulations of NK cells. CD8 forms dimer function. In most cells, CD8 is a heterodimer consisting of CD8a and CD8b, but in NK cells nearly all CD8 is homodimer of CD8a. CD8a can form co-receptor with MHC-I restricted TCR to promote T cell antigen recognition and activation.;
CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.;
CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.;
CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.

Concentration: 5 μL/Test

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

GeneID:

Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Shipping: Ice bag

Research Use Only