MUC2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66082-50, MB66082-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: Q02817

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Mucins are a family of large glycoproteins that create the structural component of mucus. Mucus functions as a blockade against pathogenic invasion and physical injury to the respiratory, gastrointestinal, and urogenital tracts. Mucins create a protective layer for epithelial cells as either membrane-bound (MUC1, MUC3, MUC16, and MUC17) or secreted (MUC2, MUC5AC, and MUC19) proteins. MUC2, or mucin-2, the main O-glycosylated protein found in mucus, is secreted by goblet cells. The structure of MUC2 contains cysteine-rich N- and C-terminal domains along with a protein core made up of heavily O-glycosylated mucin domains. The high levels of O-glycosylation along with disulfide bonding make MUC2 resistant to proteolytic cleavage. Mucin expression and glycan structure changes occur in cancers of the intestine and impact the development and progression of these cancers. The presence of MUC2 in the mucous layer of the colon helps prevent ulcerative colitis (UC) by inhibiting the invasion of bacteria.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MUC2 protein.

Bioworld Molecular Weight: ~ 540 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of MUC2 staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of MUC2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of MUC2 staining in human intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: SMUC; Mucin-2; MUC-2; Intestinal mucin-2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MUC2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified