Human CD14 (Cluster Of Differentiation 14) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1787-48, ELK1787-96, ELK1787-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Assay Type: Sandwich

Sensitivity: 0.335 ng/mL

Standard: 60 ng/mL

Detection Range: 0.94-60 ng/mL

Sample Type: serum, plasma, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD14 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P08571

Research Use Only