SARS-CoV-2 NSP10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77985-50, BS77985-100

Swiss-Prot: P0DTD1

Host: Rabbit

Reactivity: SARS-CoV-2

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: [Replicase polyprotein 1ab]: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of normal 293T cells, 293T transfected with NSP10 Protein and 293T transfected with SOST Protein, using SARS-CoV-2 NSP10 antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Bioworld Molecular Weight: 20-25KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: Recombinant fusion protein of coronavirus NSP10(YP_009725306.1).

Conjugate: Unconjugated

Modification: Unmodified