ACAP2 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71543-50, BS71543-100

Swiss-Prot: Q15057

Host: Rabbit

ACAP2 Polyclonal Antibody-BS71543

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: GTPase-activating protein (GAP for ADP ribosylation factor 6 (ARF6.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 115kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ACAP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ACAP2; CENTB2; CNT-B2

Immunogen: Recombinant fusion protein of human ACAP2(NP_036419.3).

Modification: Unmodified

Conjugate: Unconjugated