BL21(DE3) ΔserC ΔycdX Chemically Competent Cells

The BL21(DE3) ΔserC ΔycdX strain is a highly engineered E. coli host optimized for advanced protein engineering and the site-specific incorporation of non-natural amino acids. This strain is specifically designed to enhance the yield and purity of recombinant proteins containing synthetic modifications.

Technical Specifications & Mechanism

• Genotype: BL21(DE3) background with targeted deletions of both serC and ycdX genes.

• Engineered Pathway:

  • serC Deletion: Disrupts the endogenous synthesis of phosphoserine, reducing competitive interference during the translation of recombinant proteins.

  • ycdX Deletion: Removes specific phosphatase activity to further stabilize the intracellular levels of synthetic analogs.

• Primary Application: Optimized for the incorporation of phosphothreonine and phosphoserine analogs into target proteins for structural and functional analysis.

Usage Classification

• Research Use Only (RUO): This product is intended strictly for laboratory research and development purposes. It is not approved for use in clinical diagnostics, therapeutic procedures, or any human or animal applications.