Thioredoxin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91344-50, BS91344-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10599(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000
ICC:1:100-1:200
IHC:1:50-1:200

Background: Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Thioredoxin polyclonal antibody detects endogenous levels of Thioredoxin protein.

Bioworld Molecular Weight: 12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung, ICC staining TRX1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Trx, ADF, SASP, TXN, ADF, ATL derived factor, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, testicular tissue protein Li 199, THIO, Thioredoxin, thioredoxin delta 3, TRDX, TRX 1, Trx, TRX1, TXN, TXN delta 3, TXN protein, zgc:92903,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified