SERPINA1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91224-50, BS91224-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01009(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SERPINA1 polyclonal antibody detects endogenous levels of SERPINA1 protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of alpha 1 Antitrypsin on human spleen lysates using anti-alpha 1 Antitrypsin antibody at 1/1,000 dilution., ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SERPINA1, A1A, A1AT, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 antitrypsin null, Alpha 1 protease inhibitor, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, alpha1 proteinase inhibitor, Alpha1AT, Dom1, PI, PI1, PRO2275, Serine (or cysteine) proteinase inhibitor clade A member 1, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1, Serpin A1a, Serpin peptidase inhibitor clade A member 1, Serpina1, Short peptide from AAT, SPAAT, Spi1-1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified