TYMS Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91392-50, BS91392-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04818(Human) P07607(Mouse) P45352(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP, , FC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Background: Thymidylate synthase (TS), also designated TYMS, TMS, TSase and HsT422, uses 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. TS is an RNA-binding protein that can interact with its own mRNA. The TS/mRNA ribonucleoprotein complex can also associate with a number of other cellular mRNAs, including those corresponding to the p53 tumor suppressor gene and the Myc family of transcription factors. Inhibition of DNA replication and cell death resulting from thymidine depletion occurs when TS enzyme activity is inhibited with substrate or cofactor analogs, making the TS enzyme an important target for chemotherapy. Cancer cells are sensitive to thymidine depletion, as they multiply rapidly.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TYMS polyclonal antibody detects endogenous levels of TYMS protein.

Bioworld Molecular Weight: 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Thymidylate Synthase on different cell lysate using anti-Thymidylate Synthase antibody at 1/1,000 dilution.
Positive control: Lane 1: Hela
Lane 2: Jurkat
Lane 3: Raji
Lane 4: Mouse testes Lane 5: Mouse brain Lane 6: Mouse spleen, Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.

Alternative Name: TS, TSase, TYMS, OK/SW-cl.29, d TMP synthase, EC 2.1.1.45, HsT422, MGC88736, OTTHUMP00000162195, Thymidylate synthase, Thymidylate synthetase, TMS, TS, TSase, Tyms, TYMS protein, Tyms thymidylate synthetase, TYSY,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified