Fluorescein Isothiocyanate/FITC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78001-50, BS78001-100

Host: Rabbit

Reactivity: Species independent

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: The FITC rabbit polyclonal antibody are tested in Western blot against FITC conjugated Goat IgG,TRITC conjugated Goat IgG and unconjugated Goat IgG Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: Refer to figures. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Bioworld Molecular Weight: 25KDa/55KDa

Note: For research use only, not for use in diagnostic procedure.

Immunogen: FITC

Conjugate: Unconjugated

Modification: Unmodified