Amid Biosciences offers the Aldehyde Agarose Beads resins with 4% crossslinked agarose.

Aldehyde agarose beads allow the covalent binding of agarose to the amino groups of the chosen biomolecule (peptides, enzymes, antibodies and etc.). Unlike in other resins which are available for this application, the bond between the Aldehyde Agarose Beads and the biomolecule is irreversible. Aldehyde agarose beads are ready to use and require no activation.

The coupling reaction involves spontaneous formation of Schiff base bonds between aldehydes groups (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a reductant (sodium cyanoborohydride or sodium borohydride). The entire coupling reaction occurs in 2 to 6 hours in simple non-amine buffers such as PBS or borate. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20 mg of immobilized protein per milliliter of agarose resin.

Coupling efficiencies with aldehyde resin exceed those obtained with cyanogen bromide (CNBr) activated supports. Furthermore, aldehyde forms a bond with the amine-containing ligand that is more stable than with the CNBr method and is uncharged. These properties provide a more leak-resistant immobilization and lower nonspecific binding when used for affinity purification procedures.

Catalog # AA-101-5 (5 mL), AA-101-10 (10 mL).

This material is offered for research use only.