REG3G Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6908-50, BS6908-100

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100

Background: This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Extra Notes: Western blot analysis of extracts of various cell lines, using REG3G antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit. Exposure time: 10s., Immunofluorescence analysis of MCF-7 cells using REG3G antibody. Blue: DAPI for nuclear staining.

Bioworld Molecular Weight: 19kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: REG3G;LPPM429;PAP IB;PAP-1B;PAP1B;PAPIB;REG III;REG-III;UNQ429;REGIII

Immunogen: Recombinant protein of mouse REG3G.

Conjugate: Unconjugated

Modification: Unmodified